A Membrane‐Bound Prenyltransferase Catalyzes the O‐Prenylation of 1,6‐Dihydroxyphenazine in the Marine Bacterium Streptomyces sp. CNQ‐509 |
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Authors: | Philipp Zeyhle Judith S. Bauer Marco Steimle Franziska Leipoldt Manuela Rösch Prof. Dr. Jörn Kalinowski Prof. Dr. Harald Gross Prof. Dr. Lutz Heide |
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Affiliation: | 1. Pharmazeutische Biologie, Pharmazeutisches Institut, Eberhard Karls Universit?t Tübingen, Auf der Morgenstelle 8, 72076 Tübingen (Germany);2. Microbial Genomics and Biotechnology, Center for Biotechnology, Universit?t Bielefeld, Universit?tsstrasse 27, 33615 Bielefeld (Germany) |
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Abstract: | Streptomyces sp. CNQ‐509 produces the rare O‐prenylated phenazines marinophenazines A and B. To identify the enzyme catalyzing the O‐prenyl transfer in marinophenazine biosynthesis, we sequenced the genome of S. sp. CNQ‐509. This led to the identification of two genomic loci harboring putative phenazine biosynthesis genes. The first locus contains orthologues for all seven genes involved in phenazine‐1‐carboxylic acid biosynthesis in pseudomonads. The second locus contains two known phenazine biosynthesis genes and a putative prenyltransferase gene termed cnqPT1. cnqPT1 codes for a membrane protein with sequence similarity to the prenyltransferase UbiA of ubiquinone biosynthesis. The enzyme CnqPT1 was identified as a 1,6‐dihydroxyphenazine geranyltransferase, which catalyzes the C?O bond formation between C‐1 of the geranyl moiety and O‐6 of the phenazine scaffold. CnqPT1 is the first example of a prenyltransferase catalyzing O‐prenyl transfer to a phenazine. |
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Keywords: | biosynthesis enzyme catalysis phenazine prenyltransferase Streptomyces |
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