Selective DNA‐Binding by Designed Bisbenzamidine‐Homeodomain Chimeras |
| |
| Authors: | Jesús Mosquera Jéssica Rodríguez Prof. M. Eugenio Vázquez Prof. José L. Mascareñas |
| |
| Affiliation: | Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS) and Departamento de Química Orgánica, Universidade de Santiago de Compostela, Jenaro de la Fuente sn, 15782 Santiago de Compostela (Spain) |
| |
| Abstract: | We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor‐groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α‐helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction. |
| |
| Keywords: | DNA recognition molecular recognition oligonucleotides peptides supramolecular chemistry |
|
