Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) |
| |
Authors: | Daniel M Hawkins E Ann Ellis Derek Stevenson Andreas Holzenburg Subrayal M Reddy |
| |
Affiliation: | (1) School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK;(2) Microscopy & Imaging Centre, Texas A&M University, College Station, TX 77843, USA |
| |
Abstract: | We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP)
specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique
was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to
image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture
in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted
cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting
template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging
of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|