Encapsulation of BSA in polylactic acid–hyperbranched polyglycerol conjugate nanoparticles: preparation, characterization, and release kinetics |
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| Authors: | Xiujun Gao Xinge Zhang Xuejiao Zhang Cui Cheng Zhen Wang Chaoxing Li |
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| Affiliation: | (1) Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin, 300071, China; |
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| Abstract: | Nanoparticles based on an amphiphilic copolymer with polylactic acid (PLA) grafted onto hyperbranched polyglycerol (HPG) were
prepared by the use of BSA as a model protein. The characteristics of the nanoparticles were evaluated using particle size
analyzer, transmission electron microscopy, and X-ray photoelectron spectroscopy. The secondary structure of BSA released
from nanoparticles were analysed by circular dichroism experiments. Cell viability of nanoparticles was also evaluated by
using NIH 3T3 cells. The mechanism of BSA release was studied by fitting experimental data to three model equations. Results
indicated that BSA diffusion and the polymeric relaxation jointly governed the overall release process. The detailed analysis
of BSA release was performed using the first-order kinetic model equation, which gave a good fit to the experimental release
data. The influence of different copolymer structures and BSA loading capacities on release profiles were also evaluated for
the potential of using nanoparticles as controlled release protein delivery systems. |
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