| 基于上转换免疫层析技术的真菌毒素检测 |
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| 引用本文： | 於然, 马雪梅, 谢飞. 基于上转换免疫层析技术的真菌毒素检测[J]. 北京工业大学学报, 2015, 41(12): 1915-1920. DOI: 10.11936/bjutxb2015060083 |
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| 作者姓名： | 於然 马雪梅 谢飞 |
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| 作者单位： | 北京工业大学 生命科学与生物工程学院,北京,100124;北京工业大学 生命科学与生物工程学院,北京,100124;北京工业大学 生命科学与生物工程学院,北京,100124 |
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| 基金项目： | 公益性行业(农业)科研专项经费资助项目 |
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| 摘 要： | 针对目前检测脱氧雪腐镰刀菌烯醇(DON)的常用方法在检出时间或者灵敏度上都存在不足的问题,研究制备出了羧基修饰的核壳结构上转换荧光纳米颗粒,并与DON单克隆抗体偶联,应用于试纸对DON进行定量检测.对麦片中DON含量的最低检测限为0.7 ng/mL.半抑制浓度为9 ng/mL,检测范围为0.7~50.0 ng/mL,回归率在90%~156,.本研究基于上转换免疫层析技术成功建立了一种快速灵敏的检测DON的方法,可为其他包括毒素类的兽药残留检测提供借鉴.
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| 关 键 词： | 上转换荧光 免疫层析 竞争法 |
| 收稿时间： | 2015-06-29 |
Detection of Fungal Toxin Based on Up-converting Immunochtomatographic Technology |
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| WU Ran, MA Xue-mei, XIE Fei. Detection of Fungal Toxin Based on Up-converting Immunochtomatographic Technology[J]. Journal of Beijing University of Technology, 2015, 41(12): 1915-1920. DOI: 10.11936/bjutxb2015060083 |
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| Authors: | WU Ran MA Xue-mei XIE Fei |
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| Abstract: | The common methods of detection deoxynivalenol ( DON ) are limited to time-consuming or lower sensitivity. The carboxyl modification of core/shell structure up-converting nanoparticles was produced and conjugated to anti-DON antibodies, and then applied in lateral flow strip to detection DON. The detection of limitation in cornmeal was 0. 7 ng/mL. The 50% of inhibit concentration was 9 ng/mL. The detection range was from 0. 7 to 50 ng/mL and the recovery rates were from 90% to 156 %. The study has developed method for detecting DON rapidly and sensitively based on up-converting immunochromatographic technology, which can also provided reference to the detection of veterinary residues. |
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| Keywords: | up-converting fluorescence immunochromatography competitive method |
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