IspG-catalyzed positional isotopic exchange in methylerythritol cyclodiphosphate of the deoxyxylulose phosphate pathway: mechanistic implications |
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Authors: | Xiao Youli Rooker Debra You Quincy Meyers Caren L Freel Liu Pinghua |
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Affiliation: | Department of Chemistry, Boston University, Boston, MA 02215, USA. |
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Abstract: | H(2)(18)O under the bridge: Recently, the deoxyxylulose phosphate (DXP) pathway was discovered to be a second pathway supplying isoprenoid biosynthetic precursors. One of steps is an IspG-catalyzed reductive deoxygenation of methylerythritol cyclodiphosphate (MEcPP) to 4-hydroxyl-3-methyl-2-(E)-1-diphosphate (HMBPP). Using [2-(13) C,(18) O]-MEcPP, we detected the positional isotopic exchange for the bridging oxygen in MEcPP. |
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Keywords: | biosynthesis DXP pathway isoprenoids IspG positional isotopic exchange |
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