| CRISPR/Cas系统在食品质量安全检测中的应用研究进展 |
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| 引用本文： | 曹 菁,王梓莹,汪官曌,但 琨,施远国,魏 波,何庆华.CRISPR/Cas系统在食品质量安全检测中的应用研究进展[J].食品安全质量检测技术,2021,12(17):7052-7058. |
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| 作者姓名： | 曹 菁 王梓莹 汪官曌 但 琨 施远国 魏 波 何庆华 |
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| 作者单位： | 深圳大学化学与环境工程学院食品科学与工程系,深圳大学化学与环境工程学院食品科学与工程系,深圳大学化学与环境工程学院食品科学与工程系,深圳市农产品质量安全检验检测中心,深圳市农产品质量安全检验检测中心,深圳大学化学与环境工程学院食品科学与工程系,深圳大学化学与环境工程学院食品科学与工程系 |
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| 基金项目： | 深圳市科技创新委员会基础研究项目(JCYJ20200109114242138, JCYJ20180305125139107)，深圳市市场监督管理局深圳市地方标准制修订计划项目(201904, 201905)，深圳大学2020年研究生创新发展基金项目(202011) |
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| 摘 要： | 规律间隔成簇短回文重复序列(regularly spaced clustered short palindrome repeats-associated, CRISPR/Cas)系统是基于原核生物的一种适应性免疫系统, 因其可编程性和易操作的优点已被开发为基因编辑技术, 并且凭借其快速和高效的特点被广泛用于生物、医学等领域。目前, CRISPR/Cas系统已开始在基于核酸检测和单核苷酸多态性检测等食品安全检测技术中应用。本文就CRISPR/Cas系统中Cas9、Cas12a和Cas13a的原理以及其在掺假检测、溯源检测、食源性微生物和动物疫病等食品质量安全检测中的应用进展进行了综述, 以期为CRISPR/Cas系统应用于食品质量安全的快速现场检测提供理论和技术参考。
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| 关 键 词： | 规律间隔成簇短回文重复序列 Cas9 Cas12a Cas13a 食品安全检测 |
| 收稿时间： | 2021/4/26 0:00:00 |
| 修稿时间： | 2021/8/18 0:00:00 |
Research progress on the application of CRISPR/Cas system in food quality and safety detection |
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| CAO Jing,WANG Zi-Ying,WANG Guan-Zhao,DAN Kun,SHI Yuan-Guo,WEI Bo,HE Qing-Hua.Research progress on the application of CRISPR/Cas system in food quality and safety detection[J].Food Safety and Quality Detection Technology,2021,12(17):7052-7058. |
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| Authors: | CAO Jing WANG Zi-Ying WANG Guan-Zhao DAN Kun SHI Yuan-Guo WEI Bo HE Qing-Hua |
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| Affiliation: | Department of Food Science and Engineering,College of Chemistry and Environmental Engineering,Shenzhen University,Department of Food Science and Engineering,College of Chemistry and Environmental Engineering,Shenzhen University,Department of Food Science and Engineering,College of Chemistry and Environmental Engineering,Shenzhen University,Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Department of Food Science and Engineering,College of Chemistry and Environmental Engineering,Shenzhen University,Department of Food Science and Engineering,College of Chemistry and Environmental Engineering,Shenzhen University |
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| Abstract: | The clustered regularly interspaced short palindrome repeats-associated (CRISPR/Cas) system is an adaptive immune system based on prokaryotes such as bacteria and archaea. Due to its advantages of programmable and easy operation, CRISPR/Cas system has been developed as a gene editing technology, and it has been widely used in biology, medicine and other fields owing to its fast and efficient characteristics. To date, with its technical advantages of sensitivity, speed, accuracy and strong specificity, CRISPR/Cas system has been gradually widely used in nucleic acid detection, single nucleotide polymorphism detection and other food safety detection technologies. It has rapid development and broad application prospects. This paper reviewed the principles of Cas9, Cas12a, and Cas13a in the CRISPR/Cas system and summarized their application progress in food quality and safety detection, such as adulteration detection, traceability detection, food-borne microorganisms and animal diseases. The current problems and limitations were discussed. Its future development was prospected with the aim of providing theoretical and technical reference for the application of CRISPR/Cas system in the field of rapid detection of food quality and safety. |
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| Keywords: | regularly spaced clustered short palindrome repeats Cas9 Cas12a Cas13a food safety detection |
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