| Abstract: | In the past ten years there has been considerable attention devoted to improving the microbial production of cellulases for their potential in saccharification of cellulosic materials. The Rut. C-30 strain of the fungus Trichoderma reesei has yielded some of the best enzyme activities and productivities reported to date. The use of fed-batch techniques has also been shown to improve enzyme productivities while maintaining or increasing enzyme activities and reducing problems associated with aeration, mixing and foaming. In this work on fed-batch production of cellulases using Rut. C-30 the effects of both the size of the substrate injection and the time between injections are examined. The results indicate an appropriate feeding policy and provide insight into the mechanism for cellulase production. |
