| Abstract: | An improved high-performance liquid chromatography (h.p.l.c.) procedure for determination of glycoalkaloid levels in potato tubers has been developed in which Sep-Pak cartridges replace the commonly used alkaline precipitation for clean-up of tuber extracts. Glycoalkaloids are extracted from fresh tuber tissue into an aqueous medium, the extract is submitted to clean-up and α-solanine and α-chaconine are quantitatively separated on a reversed phase column with ethanolamine modifier added to the mobile phase. Potato tubers were comparatively analysed for their glycoalkaloid content by this and two other methods: a recently developed immunoassay (ELISA) and a colorimetric procedure representing the traditional chemical approach. Agreement between the h.p.l.c. and the other methods was good. |
