| CRISPR核酸检测技术的研究进展 |
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| 引用本文： | 王紫怡,黄迪,徐颖华,黄磊,连佳长,徐志南. CRISPR核酸检测技术的研究进展[J]. 食品安全质量检测学报, 2021, 12(17): 6711-6719 |
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| 作者姓名： | 王紫怡 黄迪 徐颖华 黄磊 连佳长 徐志南 |
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| 作者单位： | 浙江大学化学工程与生物工程学院,浙江大学化学工程与生物工程学院,中国食品药品检定研究院,浙江大学化学工程与生物工程学院,浙江大学化学工程与生物工程学院,浙江大学化学工程与生物工程学院 |
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| 基金项目： | 国家重点研发计划项目(2018YFC1603902)、杭州市科技发展计划项目(20202013A02) |
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| 摘 要： | 作为一种新兴的基因组编辑和调控工具,CRISPR(clustered regularly interspaced short palindromic repeats)技术的诞生和发展极大地改变了生物学领域的前进方向.近几年来,CRISPR系统及其相关蛋白(Cas效应蛋白)的工作原理被逐渐揭示,由于其优越的灵敏度和特异性...
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| 关 键 词： | CRISPR/Cas技术 Cas蛋白 核酸检测 |
| 收稿时间： | 2021-04-21 |
| 修稿时间： | 2021-06-03 |
Research progress of nucleic acid detection technology based on clustered regularly interspaced short palindromic repeats |
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| WANG Zi-Yi,HUANG Di,XU Ying-Hu,HUANG Lei,LIAN Jia-Zhang,XU Zhi-Nan. Research progress of nucleic acid detection technology based on clustered regularly interspaced short palindromic repeats[J]. Journal of Food Safety & Quality, 2021, 12(17): 6711-6719 |
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| Authors: | WANG Zi-Yi HUANG Di XU Ying-Hu HUANG Lei LIAN Jia-Zhang XU Zhi-Nan |
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| Affiliation: | College of Chemical and Biological Engineering, Zhejiang University,College of Chemical and Biological Engineering, Zhejiang University,NIFDC,College of Chemical and Biological Engineering, Zhejiang University,College of Chemical and Biological Engineering, Zhejiang University,College of Chemical and Biological Engineering, Zhejiang University |
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| Abstract: | As an emerging genome editing and regulation tool, the discovery and development of clustered regularly interspaced short palindromic repeats (CRISPR) technology have greatly changed the direction of the biological field. In recent years, the working principle of the CRISPR system and its related proteins (Cas effector proteins) have been revealed. Due to its outstanding sensitivity and specificity, this technology has also begun to play a vital role in several research fields. With its novel nuclease activity, the door was opened to develop new methods for nucleic acid detection. This article summarized the CRISPR/Cas systems and their applications and limitations in the nucleic acid detection field, and prospected for their subsequent development. With the technology maturing gradually, the CRISPR/Cas systems were expected to promote the research of basic biology and applied biology and had the potential to become a candidate for the next generation of diagnostic biosensing platforms. |
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| Keywords: | Clustered Regularly Interspaced Short Palindromic Repeats/Cas technology Cas protein Nucleic acid detection |
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