| 用核型多角病毒载体在昆虫细胞中表达人白细胞介素-4 |
| |
| 引用本文： | 薛伟钢 张春发. 用核型多角病毒载体在昆虫细胞中表达人白细胞介素-4[J]. 中国生物制品学杂志, 1996, 9(3): 117-120 |
| |
| 作者姓名： | 薛伟钢 张春发 |
| |
| 作者单位： | 卫生部长春生物制品研究所!长春130062(薛伟钢，刘东升)，国际野蚕研究中心!大连116000(张春发) |
| |
| 摘 要： | 将编码人白细胞介素-4（IL－4）的cDNA插入苜蓿银纹夜蛾单核多角病毒（Autogranhacalifor－nicanuclearpolyhedrosisvirus，AcNPV）的多角体蛋白基因启动子下游，构建表达IL－4的重组病毒。感染该重组病毒的细胞可表达较高水平的IL－4多肽。所表达的重组IL－4具有较高的生物学活性，分子量为16KDa。
|
| 关 键 词： | 人白介素-4 昆虫核型多角病毒 基因克隆 基因表达 |
Expression of Human IL-4 in Insect Cell Using Nuclear Polyhedral Virus Vector |
| |
| Xue Weigang, Liu Dongsheng et al. Expression of Human IL-4 in Insect Cell Using Nuclear Polyhedral Virus Vector[J]. Chinese Journal of Bilogicals, 1996, 9(3): 117-120 |
| |
| Authors: | Xue Weigang Liu Dongsheng et al |
| |
| Abstract: | A recombinant virus strain was constructed by inserting the cDNA coding hu-man IL-4 into the end of polyhedral protein gene promotor of autographa californica nuclear polyhedrosis virus (Ac NPV). The expression level of IL-4 in the cell infected with the virus strain was relatively high, and the expressed IL-4, with a molecular weight of 16KDa,showed high biological activity. |
| |
| Keywords: | Insect nuclear polyhedral virus IL-4 Gene expression |
| 本文献已被 CNKI 维普 等数据库收录！ |
