| 人活化素结合蛋白的分离及纯化 |
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| 引用本文： | 柳忠辉,汪丽.人活化素结合蛋白的分离及纯化[J].粉末涂料与涂装,1996,9(4):152-155. |
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| 作者姓名： | 柳忠辉 汪丽 |
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| 作者单位： | 白求恩医科大学基础医学院!长春130021(柳忠辉，杨煜，王树和)，白求恩医科大学七年制医学系(姚勇)，白求恩医科大学第三临床学院!长春130021(汪丽) |
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| 摘 要： | 为了进一步研究人活化素结合蛋白的免疫生物学活性，从人卵泡液中提取了活化素结合蛋白，采用亲和层析及高效液相层析法，最终获得35KDa，SDS－PAGE显示为一条带的人活化素结合蛋白，经免疫活性测定、激活素结合试验以及FSH分泌抑制试验证实，所纯化的人活化素结合蛋白为具有生物学活性的天然活化素结合蛋白，可供进一步的免疫生物学研究用。
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| 关 键 词： | 活化素结合蛋白 生物学活性 |
Separation and Purification of Human Follistatin |
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| Liu Zhonghui, Yang Yu, Yao Yong et al.Separation and Purification of Human Follistatin[J].Chinese Journal of Biologicals,1996,9(4):152-155. |
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| Authors: | Liu Zhonghui Yang Yu Yao Yong |
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| Abstract: | In order to study on the blological activity of follistatin,it was extracted from human follicular fluul and purified by affinity and high performance liquid chromatographies.SDS-PAGE profile showed that the final product was a single band with a molecular weight of 35KDa. Immunological activity detection,activin binding test and FSH secretion inhibition test proved that the final product was a natural biologically-active follistatin and further study on its immunobiology is needed. |
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| Keywords: | Follistatin Biological activity |
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