盐藻胞外多糖分离纯化方法研究

盐藻培养液经离心、超滤浓缩得胞外多糖粗品溶液,然后用乙醇沉淀,丙酮及无水乙醚洗涤,并冷冻干燥得胞外多糖（Exopolysaccharides,EPS）粗品。粗糖经双酶解、透析等初步纯化后,用DE-52离子交换柱层析分离纯化得两种EPS组分（EPSⅠ和EPSⅡ）,琼脂糖凝胶电泳鉴定各自为均一的成分。经检测,两组分的糖质量分数以葡聚糖为对照分别为61.34%和52.81%,蛋白质质量分数3.97%和1.35%,且核酸质量分数小于0.025%,达到了比较高的纯度。

Separation and Purification of Extracellular Polysaccharides from Dunaliella salina

The crude solution containing extracellular polysaccharide was obtained by centrifugation and ultrafiltration from the Dunaliella salina culture, followed by the ethanol precipitation, washing with acetone and anhydrous ether and freeze drying. After the primary purification using the double-enzyme hydrolyzation and the dialysis, two polysaccharide components (EPS I and EPS II), which were identified using the agarose gel electrophoresis, were purified by the DE-52 ion-exchange chromatography. As a result, the polysaccharide contents of EPS I and EPS II were achieved at 61.34% and 52.81%, and the protein contents were 3.97% and 1.35%, respectively. But the nucleic acid contents were decreased and low than 0.025%. This resucts indicated that a high purity of polysaccharide was gained..