富勒醇的羟基活化及其产物对蛋白修饰

考察了2-氟-1-甲基吡啶对甲苯磺酸盐(FMP)对富勒醇羟基的活化方法,以便将富勒醇通过共价交联的方法与牛血清白蛋白(BSA)连接,进而研究富勒醇修饰对BSA所造成的影响。富勒醇的合成以富勒烯为原料,以四丁基氢氧化铵(TBAH)为相转移催化剂,在NaOH为催化剂的作用下进行;FMP对富勒醇羟基的活化以三乙胺为催化剂;富勒醇的表征用紫外吸收光谱和红外光谱分析的方法;富勒醇对BSA修饰后的产物的纯化和检测分别用凝胶过滤柱和SDS-PAGE电泳检测进行。富勒醇的红外光谱在3000cm~(-1)处有宽的羟基吸收峰以及碳碳双键、碳环、碳碳单键、碳氢单键等富勒醇的特征吸收峰。富勒醇对BSA修饰后,引起BSA在280nm处吸光度升高。结果表明,FMP可以对富勒醇上的羟基进行活化进而是富勒醇进行修饰。

ACTIVATION OF HYDROXYL GROUPS IN FULLERENOL AND ITS APPLICATION IN MODIFICATION OF PROTEINS

The method of activating hydroxyls of fullerenols using 2-fluoro-l-methylpyridinium p-tolu- ene sulphonate (FMP) to covalently linking with bovine serum albumin (BSA) and further its effect on BSA was studied.The fullerene was the material for the synthesis of fullerenols,tetrabutyl ammo- nium hydroxide (TBAH) was phase-transfer solvent and NaOH was the catalyst in the synthesis of fullerenols.Triethylamine was used as catalyst for the activation of the hydroxyls of the fullerenol by FMP.The characterization of the fullerenols was carried out by UV adsorption and IR.The purifica- tion and examination of the modified product was carried out with gel filtration chromatography.The IR spectrum showed characteristic adsorption peaks of the hydroxyl,C=C,C-cycle,C-C and C-H in fullerenols.The BSA modified with fullerenols caused the increase in the intensity at 280 nm.The results suggested that the hydroxyls of the fullerenols could be activated by FMP and further used for the modification of BSA.