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亚麻籽胶中酸性多糖和中性多糖的分离纯化
引用本文:陈海华,许时婴,王璋.亚麻籽胶中酸性多糖和中性多糖的分离纯化[J].食品与发酵工业,2004,30(1):96-100.
作者姓名:陈海华  许时婴  王璋
作者单位:江南大学食品学院,无锡,214036
摘    要:采用十六烷基三甲基溴化胺 (CTAB)络合法 ,利用离子交换柱层析和凝胶柱层析从亚麻籽胶中分离得到酸性多糖 (AFM -1 )和中性多糖 (NFM -1 )纯品 ,其分子质量分别为 7 62× 1 0 5u和 1 1 9× 1 0 6u ,总糖含量分别为 5 8 92 %和 84 93%,糖醛酸含量分别为 33 5 5 %和 6 5 8%,AFM-1中C、H和N的含量分别为 2 8 0 4%、5 89%和 0 80 %,红外光谱测定表明 ,AFM -1和NFM -1具有多糖的特征吸收 ,并且其糖环均为吡喃环。

关 键 词:亚麻籽胶  中性多糖  酸性多糖  分离  纯化

Separation and Purification of Acidic Polysaccharides and Neutral Polysaccharides from Flaxseed Gum
Chen Haihua,Xu Shiying,Wang Zhang.Separation and Purification of Acidic Polysaccharides and Neutral Polysaccharides from Flaxseed Gum[J].Food and Fermentation Industries,2004,30(1):96-100.
Authors:Chen Haihua  Xu Shiying  Wang Zhang
Abstract:AFM-1 and NFM-1 were obtained using CTAB-Complex, ion-exchange and gel-filtration method. It is shown that their molecular weight were 7.62×10 5 and 1.19×10 6, total sugar contents were 58.92% and 84.93% and uronic acid contents were 33.55% and 6.58%, respectively. The C, H, N contents of AFM-1 were 28.04%, 5.89%, 0.80%. The structure of both AFM-1 and NFM-1 contain pyranride and has the characteristic of glycoconjugate.
Keywords:flaxseed gum  neutral polysaccharide    acidic polysaccharide    separate  purification
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