Prevention of cholesterol cholelithiasis by dietary unsaturated fats in hormone-treated female hamsters |
| |
Authors: | Nariman Ayyad Bertram I Cohen Akira Ohshima Erwin H Mosbach |
| |
Affiliation: | (1) Albert Einstein College of Medicine, Bronx, New York;(2) Department of Surgery, Beth Israel Medical Center, First Avenue at 16th St., 10003 New York, NY;(3) Present address: Department of Surgery 1, Kyushu University School of Medicine, Maidashi, 3-1-1, Higashi-ku, 812 Fukuoka, Japan |
| |
Abstract: | We examined the effect of diet on gallstone incidence and the composition of biliary phosphatidylcholines in methyltestosterone-treated
female hamsters. These hamsters were fed a nutritionally adequate purified lithogenic diet containing 2% corn oil, 4% butterfat,
0.3% cholesterol, and 0.05% methyltestosterone, resulting in a cholesterol gallstone incidence of 86%. This incidence was
lowered when mono-and polyunsaturated fats or fatty acids were added to the diet: 2.5% oleic acid resulted in total prevention
of cholesterol cholelithiasis, 2.5% linoleic acid, and 4% safflower oil (78% linoleic acid content) reduced gallstone incidence
to 26 and 8%, respectively. An additional 4% butterfat (29% oleic acid content) produced gallstones in 50% of the animals.
At the end of the 6-wk feeding period, the bile of all hamsters was supersaturated with cholesterol. The major biliary phosphatidylcholine
species in all groups were (sn-1-sn-2): 16:0–18:2, 16:0–18:1, 18:0–18:2, 16:0–20:4, and 18:2–18:2. The safflower oil-and linoleic acidfed hamsters exhibited
an enrichment of 16:0–18:2 (16–18%); added butterfat or oleic acid increased the proportion of 16:0–18:1 (9 and 25%, respectively).
We conclude that the phosphatidylcholine molecular species in female hamster bile can be altered by dietary fats/fatty acids
and that mono-and polyunsaturated fatty acids play a role in suppressing the induced cholelithiasis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|