| 电离辐射诱导HeLaS_3细胞凋亡时程变化的影响 |
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| 引用本文： | 金光辉,薛丽香,付士波,苏旭.电离辐射诱导HeLaS_3细胞凋亡时程变化的影响[J].辐射研究与辐射工艺学报,2001(4). |
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| 作者姓名： | 金光辉 薛丽香 付士波 苏旭 |
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| 作者单位： | 白求恩医科大学卫生部放射生物重点实验室 长春130021
(金光辉，薛丽香，付士波)，白求恩医科大学卫生部放射生物重点实验室 长春130021(苏旭) |
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| 基金项目： | 卫生部科学研究基金 (98- 1- 2 0 2 )资助 |
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| 摘 要： | 采用PI和Hoechst33342双染、流式细胞术 (FCM)和图像分析术 (ICM)检测了不同剂量电离辐射对HeLaS3 细胞凋亡的影响。结果表明 ,HeLaS3 细胞在分别受到 0 .5 - 4 .0GyX射线照射后 8h ,其细胞凋亡呈增高趋势 ,但无统计学意义。照射后 12h ,细胞凋亡显著增加 (p <0 .0 1) ,并达峰值。随后 ,细胞凋亡率迅速下降 ,4 8h ,又有所回升。且流式细胞术与图像分析术所得结果数据相近 ,趋势一致。
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| 关 键 词： | 电离辐射 细胞凋亡 流式细胞术 图像分析术 |
INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS_3 CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS |
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| JIN Guanghui,XUE Lixiang,FU Shibo,SU Xu.INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS_3 CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS[J].Journal of Radiation Research and Radiation Processing,2001(4). |
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| Authors: | JIN Guanghui XUE Lixiang FU Shibo SU Xu |
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| Abstract: | Changes of time course in apoptosis of HeLaS 3 cells following irradiation with different doses X rays were observed. The percentages of apoptotic cells were examined by both flow cytometry (FCM) and image cytometry (ICM) after the cells were stained by propidium iodine (PI) and Hoechst33342 (HO). It was founded that the percentages of apoptotic cells increased after irradiation with 0.5-4.0Gy X rays and the peak value emerged at the 12th hour after irradiation .In additon the values examined by both FCM and ICM were nearly same and the tendency was identical. So irradiation may induce apoptosis of HeLaS 3 cell lines. |
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| Keywords: | Irradiation Apoptosis Flow cytometry Image cytometry |
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