IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization |
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Authors: | D van Soolingen J Bauer V Ritacco SC Le?o I Pavlik V Vincent N Rastogi A Gori T Bodmer C Garzelli MJ Garcia |
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Affiliation: | Diagnostic Laboratory of Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands. D.van.Soolingen@rivm.nl |
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Abstract: | Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered. |
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