深层发酵生产红菇菌丝体和多糖的培养基优化研究

研究了采用液体发酵法生产红菇菌丝体及红菇多糖的发酵培养基.研究了碳源、氮源和金属离子对红菇细胞生长及红菇多糖的影响.结果表明：葡萄糖作为最佳碳源有利于细胞生长和胞内外多糖等代谢产物的积累而获得高的多糖产率;酵母膏作为最适氮源能获得最高的菌丝生物量、胞外多糖产量和多糖产率,尽管此时胞内多糖处于一个稍低水平;金属离子也对细胞生长具有影响,在锌离子存在的情形下能够获得最佳的细胞生长及代谢产物累积效果.在最优化培养基的条件下,细胞干重、胞外多糖、胞内多糖、总多糖及多糖产率分别达到20.94g/L,1.54g/L,78.11mg/g,2.95g/L和8.13%.

Optimization of Medium for Mycelia Growth and Polysaccharides Production by Submerged Fermentation of Russula sp.

A submerged fermentation medium of Russula sp.was developed for efficient production of polysaccharide and biomass. The effects of carbohydrate and nitrogen sources and cations on the Russula fermentation were investigated in shake flasks. It was found that glucose was favorable carbohydrate sources for cell growth, metabolite production of extra cellular polysaccharide (EPS) and intracellular polysaccharide (IPS), the yield of polysaccharides per unit biomass. Yeast extract as nitrogen sources was suitable for the cell growth, extra cellular polysaccharide production and the yield of polysaccharides although intracellular polysaccharide was at a lower level. The type of cations source also affects polysaccharide production, and best results were obtained with zinc chloride. The optimal production of biomass, EPS, IPS, TPS and yield of polysaccharides was 20.94g/L, 1.54g/L, 78.11mg/g, 2.95g/L and 8.13%, respectively.