| 碱性淀粉酶菌株的紫外线和He-Ne激光复合诱变及其产酶条件的研究 |
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| 引用本文： | 刘建龙,刘建军,杨连生.碱性淀粉酶菌株的紫外线和He-Ne激光复合诱变及其产酶条件的研究[J].四川激光,2005,26(3):83-84. |
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| 作者姓名： | 刘建龙 刘建军 杨连生 |
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| 作者单位： | 华南理工大学;华南理工大学 |
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| 基金项目： | 山东省科技攻关计划课题 (95 0 7) |
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| 摘 要： | 以嗜碱芽胞杆菌BC -A3 6为出发菌株,通过紫外线(UV)、亚硝基胍(NTG)及激光复合诱变处理,选育出一株目前高产碱性淀粉酶的突变株JG -5 7,在摇瓶发酵培养基上3 5℃培养45h ,测得酶活力稳定在867u/ml左右,为出发菌株酶活力的2 .1倍。该菌株产酶适宜的pH值为9-10。
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| 关 键 词： | 嗜碱芽胞杆菌 激光复合诱变 碱性淀粉酶 产酶条件 |
| 文章编号： | 0253-2743(2005)03-0083-02 |
| 修稿时间： | 2004年4月5日 |
Study on the breeding of alkaline amylase producing strain by uv and he-ne laser compound mutation and the conditions for enzyme production |
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| LIU Jian-long,LIU Jian-jun,YANG Lian-sheng.College of Food and Bilogical Engineering,South China University of Technology,Guangzhou China,.Food and Fermentation Key Laboratory of Shandong Province,Jinan China.Study on the breeding of alkaline amylase producing strain by uv and he-ne laser compound mutation and the conditions for enzyme production[J].Laser Journal,2005,26(3):83-84. |
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| Authors: | LIU Jian-long LIU Jian-jun YANG Lian-shengCollege of Food and Bilogical Engineering South China University of Technology Guangzhou China Food and Fermentation Key Laboratory of Shandong Province Jinan China |
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| Affiliation: | LIU Jian-long1,LIU Jian-jun2,YANG Lian-sheng11.College of Food and Bilogical Engineering,South China University of Technology,Guangzhou 510641 China,2.Food and Fermentation Key Laboratory of Shandong Province,Jinan 250014 China) |
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| Abstract: | |
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| Keywords: | alkalophilic bacillus laser compound mutation alkaline amylase enzyme production conditions |
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