Label-free sensitive optical detection of polychlorinated biphenyl (PCB) in an aqueous solution based on surface plasmon resonance measurements |
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| Authors: | Surin Taewook Seogil Jungwoo Inhee Kyunghee Jongheop |
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| Affiliation: | aSchool of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 151-742, Republic of Korea;bDepartment of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea;cNational Institute of Environmental Research, Republic of Korea |
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| Abstract: | A label-free, ultrasensitive method for the optical detection of polychlorinated biphenyl (PCB) is described. The detection mechanism for PCB is based on PCB-induced conformational changes of immobilized Cytochrome c (Cyt c) on an Au thin film altering the local dielectric function of the supported Cyt c, which can be detectable by surface plasmon resonance (SPR) spectroscopy. Results showed that the confirmations of Cyt c changed in a very sensitive way depend on PCB concentrations as confirmed by circular dichroism (CD) measurements and atomic force microscope (AFM) observations. Based on the results, we investigated the PCB-sensitive detection performance of the self-assembling Cyt c monolayer surface by SPR. On exposure to PCB, the reflectance R at the SPR angle of the supported Cyt c showed a sensitive and systematic increase with increasing PCB concentrations. The limit of detection was as low as 0.1 ppb and the responses completed within 10 min. |
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| Keywords: | Biosensor Conformational change Cytochrome c Polychlorinated biphenyl Self-assembly Surface plasmon resonance |
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