| 分光光度法测定甜蜜素 |
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| 引用本文： | 桑宏庆,王丽,王光新. 分光光度法测定甜蜜素[J]. 中国调味品, 2011, 36(3) |
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| 作者姓名： | 桑宏庆 王丽 王光新 |
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| 作者单位： | 安徽科技学院,安徽,凤阳,233100 |
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| 基金项目： | 安徽科技学院自然科学课题资助项目 |
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| 摘 要： | 目的:探讨分光光度法测定甜蜜素的方法.方法:将甜蜜素中的氮转化为铵,在碱性条件下与水杨酸钠-次氯酸钠反应生成蓝色物质,用分光光度法测定吸光度进行定量.结果:吸光值与甜蜜素的含量在一定浓度范围内成正比,最大吸收波长为658 nm,甜蜜素在0～16 μg/mL范围内符合比耳定律,相对标准偏差2.74%～3.94%,平均回收率为94.12%～96.04%.结论:方法准确可靠、易于掌握,适宜饮料、蜜饯等食品的测定.
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| 关 键 词： | 甜蜜素 水杨酸钠 次氯酸钠 分光光度法 |
Determination of sodium cyclamate by spectrophotometry |
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| SANG Hong-qing,WANG Li,WANG Guang-xin. Determination of sodium cyclamate by spectrophotometry[J]. China Condiment, 2011, 36(3) |
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| Authors: | SANG Hong-qing WANG Li WANG Guang-xin |
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| Affiliation: | SANG Hong-qing,WANG Li,WANG Guang-xin(Anhui Science and Technology University,Fengyang 233100,China) |
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| Abstract: | Objective:To explore the determination method of saccharin in food by spectrophotometry.Methods:The nitrogen in the sodium cyclamate was transformed into ammonium ion by being digested,and then it reacted with sodium salicylate-sodium hypochlorite under the alkalinity conditions.The blue material that was produced in this reaction was determined by spectrophotometry.Results:The absorbance of sample solution was proportional with the concentration of cyclamate in a certain range.The maximum absorption wavele... |
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| Keywords: | sodium cyclamate sodium salicylate sodium hypochlorite spectrophotometry |
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