液质联用技术检测祛痘类化妆品中西咪替丁等21种非法添加药物

建立了祛痘类化妆品中西咪替丁、四环素类、喹诺酮类等21种禁用物质的液质联用检测方法.以饱和氯化钠分散样品,质量分数0.5％甲酸-乙腈提取,-20℃冷冻10 min,10000 r/min冷冻离心取上清液过滤后测定.以质量分数0.1％甲酸乙腈-0.1％甲酸水溶液作为流动相梯度洗脱,流速为0.4 mL/min.采用正离子模...

Determination of Twenty-One Illegal Drugs Including Cimetidine in Anti-Acne Cosmetics by Liquid Chromatography-Tandem Mass Spectrometry

A method for the determination of 21 illegal drugs including cimetidine,tetracycline and quinolone in anti-acne cosmetics by liquid chromatography-tandem mass spectrometry was established.The sample was separated with saturated sodium chloride solution,extracted with 0.5%(mass fraction)formic acid-acetonitrile,frozen at-20℃for 10 min,centrifuged at 10000 r/min and finalized filtration to conduct detection.The flow rate was 0.4 mL/min with 0.1%(mass fraction)acetonitrile formate-0.1%formic acid solution as mobile phase by gradient elution.Positive ion mode electrospray ionization(ESI⁺),multireaction monitoring(MRM)mode detection and matrix matching standard curve external standard method were used.The results showed a good linear relationship(r>0.99)for all samples,the detection limits were 0.001-0.02μg/g,the recoveries were 73.9%-115.6%,and RSDs were 1.1%-10.9%.This method can provide technical support for daily supervision of anti-acne cosmetics.