从虎杖中同时提取分离白藜芦醇和蒽醌类工艺研究

以自然发酵后的虎杖为原料,采用甲醇热回流同时提取虎杖中的白藜芦醇和蒽醌类化合物,选择甲醇浓度、料液比、提取次数和提取时间作为考察因素,以白藜芦醇和蒽醌类化合物的含量为指标,通过正交试验,得到白藜芦醇和蒽醌类化合物同时提取的适宜条件为:体积分数98%甲醇,料液比1∶6,提取3次,每次1 h,白藜芦醇的含量1.912%,提取率达90%,蒽醌类化合物的含量为1.504%,提取率达91.15%;粗提物经V(丙酮)∶V(乙醚)=1∶3的混合溶剂纯化,体积分数50%乙醇溶解分离后,分别得到了白藜芦醇和蒽醌粗品,粗品中白藜芦醇的含量达63.72%,蒽醌含量达50.82%。

Study on Extraction and Separation of Resveratrol and Anthraquinone from Polygonum cuspidatum Sieb.et.Zucc

Resveratrol and anthraquinone were extracted and separated from Polygonum cuspidatum Sieb.et.Zucc.Methanol hot reflux orthogonal test was applied.Mixed solvent was used in purification and 50% ethanol was used in separation.The optimum conditions of extraction were: 98% methanol,solid-liquid ratio 1∶ 6,extracted 3 times and 1h/ each time.As a result,the content of resveratrol was 1.912% and the content of anthraquinone was 1.504%.The extraction efficiency of resveratrol was 90%,and the extraction efficiency of anthraquinone was 91.15%.Acetone:ether 1∶ 3 was used in purifying,then 50% ethanol was used to separate.Resveratrol and anthraquinone content in coarse product was 63.72% and 50.82%,respectively.This study provided experimental evidence for comprehensive development and utilization of Polygonum cuspidatum Sieb.et.Zucc.