Cloning and primary structure of the chiA gene from Aeromonas caviae |
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Authors: | Y Sitrit CE Vorgias I Chet AB Oppenheim |
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Affiliation: | Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel. |
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Abstract: | The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane. |
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