紫外分光光度法测定维多康中维生素C的含量

目的:建立紫外分光光度法直接测定维多康中维生素C含量的方法。方法:以Cu2+为催化剂加速维生素C的氧化,以EDTA络合校正背景作参比,利用氧化前后吸光度差值(△A)直接测定维生素C的含量。测得还原型维生素C在265nm处有最大吸收峰。结果:维生素C的浓度在30～70μg/ml范围内吸光度与浓度呈良好的线性关系r=0.99922(n=5),回收率在105.77%～109.50%之间。结论:操作简单,测定快速,结果准确,精密度高,检测范围大,适合于维多康中维生素C的含量测定。

Determination of Ascorbic Acid in Weiduokan by Ultraviolet Spectrophotometry

Objective: To establish an Ultraviolet Spectrophotometry method for the determination of ascorbic acid in theweiduokang.Method:Using Cu2+as the catalyst to accelerate the oxidation of VC EDTA .resmbling correction background ascomparison .By mean of easuring the difference ( A) of adsorbency before and after the oxidation to obtain the content of VC. Itshowed that there exists a maximum obsorption peak of reduction type VC at the position of 265.2nm.Results:The calibration curveof ascorbic acid was linear (r=0.99922) over the recovery is between 105.77%～109.50%.Conclusion:The method is sensitive,accurate, precision is high, can be applied to determine ascorbic acid in other preparations.