Synthesis and Antitumor Activity of Ether Glycerophospholipids Bearing a Carbamate Moiety at the sn‐2 Position: Selective Sensitivity Against Prostate Cancer Cell Lines |
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Authors: | Hoe‐Sup Byun Dr. Robert Bittman Prof. Pranati Samadder Dr. Gilbert Arthur Prof. |
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Affiliation: | 1. Department of Chemistry and Biochemistry, Queens College of The City University of New York, Flushing, NY 11367‐1597 (USA), Fax: (+1)?718‐997‐3349;2. Department of Biochemistry and Medical Genetics, University of Manitoba, 745 Bannatyne Avenue, Winnipeg, Manitoba R3E?0J9 (Canada), Fax: (+1)?204‐789‐3421 |
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Abstract: | Analogues of 1‐O‐hexadecyl‐sn‐3‐glycerophosphonocholine (compounds 1 – 4 ) or sn‐3‐glycerophosphocholine (compound 5 ) bearing a carbamate or dicarbamate moiety at the sn‐2 position were synthesized and evaluated for their antiproliferative activity against cancer cells derived from a variety of tissues. Although all of the compounds are antiproliferative, surprisingly the carbamates ( 1 and 2 ) are more effective against the hormone‐independent cell lines DU145 and PC3 than toward other cancer cell lines we examined. This selectivity was not observed with the dicarbamates ( 3 and 4 ). Phosphocholine carbamate analogue 5 is as effective against the prostate cancer cell lines as the corresponding phosphonocholine analogue 1 . Cell death induced by 2′‐(trimethylammonio)ethyl 4‐hexadecyloxy‐3(R)‐N‐methylcarbamoyl‐1‐butanephosphonate (carbamate analogue 2 ) appeared to be mediated by apoptosis, as assessed by caspase activation and loss of mitochondrial membrane potential. The in vivo activity of 2 was evaluated in a murine prostate cancer xenograft model. Oral and intravenous administration showed that 2 is effective in inhibiting the growth of PC3 tumors in Rag2M mice. Our studies show that the glycerolipid carbamates reported herein represent a class of prostate‐cancer‐selective cytotoxic agents. |
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Keywords: | antitumor agents cytotoxic lipids glycerophosphonocarbamates medicinal chemistry prostate cancer |
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