Analysis of flavonoids and phenolic acids in Greek aromatic plants: Investigation of their antioxidant capacity and antimicrobial activity |
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Authors: | C. Proestos I.S. Boziaris G.-J.E. Nychas M. Komaitis |
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Affiliation: | 1. Laboratory of Food Chemistry, Food Science and Technology Department, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece;2. Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece |
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Abstract: | The phenolic fraction of plant extracts has been linked to their antioxidant capacity and antimicrobial activity. In the present paper, RP-HPLC with UV detection was employed to distinguish phenolic compounds from the other constituents of the plants examined. The phenolic substances were identified and quantified after comparison with reference standards. A GC–MS method is also presented for characterization of different phenolics as trimethylsilyl derivatives. The antioxidant capacity was determined, in dried plants and in their methanol extracts, with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenolic content in the extracts was determined spectrometrically applying the Folin-Ciocalteu assay. It ranged from 2.9 to 28.2 mg gallic acid/100 g dry sample. Antimicrobial activity of the extracts against selected microorganisms was also investigated. |
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Keywords: | Phenolic antioxidants RP-HPLC GC&ndash MS Trimethylsilyl derivatives Antimicrobial activity |
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