Screening of antioxidative properties of the methanolic extracts of Pelargonium endlicherianum Fenzl., Verbascum wiedemannianum Fisch. & Mey., Sideritis libanotica Labill. subsp. linearis (Bentham) Borm., Centaurea mucronifera DC. and Hieracium cappadocicum Freyn from Turkish flora |
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Authors: | Bektas Tepe Munevver Sokmen H Askin Akpulat Onder Yumrutas Atalay Sokmen |
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Affiliation: | 1. Cumhuriyet University, Faculty of Science and Literature, Department of Biology, 58140 Sivas, Turkey;2. Cumhuriyet University, Faculty of Science and Literature, Department of Chemistry, 58140 Sivas, Turkey |
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Abstract: | Five extracts from five plants, of which four are endemic to Turkish flora, were screened for their possible in vitro antioxidant activities by two complementary test systems, namely DPPH free radical-scavenging and β-carotene/linoleic acid. In the first case, Pelargonium endlicherianum extract exerted greater antioxidant activity than those of other plant extracts studied with an IC50 value of 7.43 ± 0.47 μg/ml, followed by Hieracium cappadocicum (30.0 ± 0.14 μg/ml). When compared to the synthetic antioxidant BHT (18.0 ± 0.40 μg/ml), the methanolic extract of P. endlicherianum exhibited more than two fold greater antioxidant activity. In the β-carotene/linoleic acid test system, the most active plant was P. endlicherianum with 72.6% ± 2.96 inhibition rate, followed by H. cappadocicum (55.1% ± 2.33) and Verbascum wiedemannianum (52.5% ± 3.11). Antioxidant activities of curcumin and ascorbic acid were also determined as positive controls in parallel experiments. |
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Keywords: | Turkish medicinal plants Antioxidant activity Methanolic extract BHT Curcumine Ascorbic acid |
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