Purification and characterization of soluble invertases from suspension-cultured bamboo (Bambusa edulis) cells |
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Authors: | Chia-Chen Liu Li-Chun Huang Chen-Tien Chang Hsien-Yi Sung |
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Affiliation: | 1. Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC;2. Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, ROC;3. Department of Food and Nutrition, Providence University, Shalu, Taiwan, ROC |
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Abstract: | An alkaline invertase (IT I) and an acid invertase (IT II) were purified from the soluble fraction of suspension cultured bamboo cells. Both purified invertases were homogeneous as examined by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) and were identified as β-fructofuranosidases able to attack the β-fructofuranoside from the fructose end. With respect to sucrose hydrolysis, the optimal pHs were 7.0 and 4.5 for IT I and IT II, respectively. The Km’s were 10.9 and 3.7 mM. The IT I and IT II molecular masses were 240 and 68 kDa, respectively, as estimated by gel filtration. The isoelectric points were 4.8 and 7.4. IT I was a homotetrameric enzyme activated by bovine serum albumin (BSA). IT II was a monomeric enzyme activated by BSA, concanavalin A (ConA) and urease. Both isoforms were significantly inhibited by heavy metal ions Ag+ (5 mM) and Hg2+ (1 mM), and mercaptide forming agent ρ-chloromercuribenzoic acid (PCMB; 0.5 mM). |
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Keywords: | Bamboo (Bambusa edulis) suspension cells Invertase Purification Characterization |
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