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Characterization of spatial cell distribution in multilayer sheet of human keratinocytes through a stereoscopic cell imaging system |
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| Authors: | Kim Mee-Hae Tsubakino Naoki Kagita Shogo Taya Masahito Kino-oka Masahiro |
| Affiliation: | 1Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan;2Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan |
| Abstract: | A novel system was developed to characterize the spatial distribution of human keratinocytes in cultured epithelial sheet, based on stereoscopic observation of nuclei using a confocal laser microscope in combination with image processing. The presented technique allowed understanding of the proliferative and growth-arrested states of cells in multilayered sheet. |
| Keywords: | Keratinocyte sheet Stratification Proliferative and growth-arrested cells Basal layer stability Spatial cell distribution Stereoscopic image processing Regenerative medicine |
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