Platelet-activating factor detected in bronchoalveolar lavage fluids from an asthmatic patient |
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Authors: | Tsugio Horii Hitoshi Okazaki Minoru Kino Yohnosuke Kobayashi Kiyoshi Satouchi Kunihiko Saito |
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Affiliation: | (1) Department of Pediatrics, Kansai Medical University, 570 Moriguchi, Osaka, Japan;(2) Department of Medical Chemistry, Kansai Medical University, 570 Moriguchi, Osaka, Japan;(3) Department of Food Science and Technology, Faculty of Engineering, Fukuyama University, 729-02 Fukuyama, Japan |
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Abstract: | It recently has been recognized that platelet-activating factor (PAF) may be a mediator of asthma exacerbation. We had the
opportunity to analyze bronchoalveolar lavage fluids from an asthmatic infant, which were characterized by neutrophil infiltration.
The patient's lungs were washed on three occasions with saline during asthmatic attacks. PAF was found in each case on the
basis of its ability to cause the immediate aggregation of washed rabbit platelets. The PAF detected was equivalent to 1–1.4
pmol of 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine, three quarters of which were recovered in cell-associated form. By contrast, we did not detect
PAF in bronchoalveolar exudates from patients with larungeal stenosis or with respiratory distress syndrome. LysoPAF, the
direct precursor as well as initial metabolite of PAF, was also analyzed after being converted to PAF by acetylation. There
was a wide variation in the amount of lysoPAF present in individual patients, suggesting that lysoPAF levels cannot be taken
as an indicator for the presence of PAF.
Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl
Ether Lipids, Tokyo, Japan, May 1989. |
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