食品中硝酸盐酶法快速检测试纸条的设计与应用

目的:对食品中硝酸盐酶法快速检测试纸条进行了设计并应用于几种蔬菜进行验证。方法:用2.0%的N-1-萘基乙二胺二盐酸、3.0%的对氨基苯磺酸和6.0%酒石酸溶液按体积1:1:1混合作为显色剂,利用定量滤纸为试纸条载体和硝酸盐还原酶为还原剂制作了酶法快速检测试纸条并与国家标准法对比检测了几种蔬菜中硝酸盐含量。结果:利用显色剂和硝酸盐还原酶还原剂与硝酸盐发生的显色反应可以实现对硝酸盐的快速检测。结论:该试纸条能够比较快速(1min)地对食品中的硝酸盐进行检测且准确度高、灵敏度高、体积小、成本低廉,开发潜力巨大。

Designing and Application of Test Strip for Fast Detection of Nitrate in Food Based on Enzymatic Method

Objective:A test strip for fast detection nitrate in food was designed based on enzymatic method and applied in several vegetables. Methods:With 2.0% N-1-naphthylethylenediamin dihydrochloride,3.0% sulfanilic acid and 6.0% tartaric acid mixed by the volume 1:1:1 as the color-developing reagent,qualitative filter paper used for the test strip carrier and nitrate reductase used for the reductant,the test strip was made successfully and used to detect nitrate levels in several vegetables compared with national standard method of China. Results:the color-developing reagent and nitrate reductase could react for fast detection of nitrate. Conclusion:The designed test strip can be used to detect nitrate in food,with higher precision and sensitivity,smaller volume and lower cost,therefore it would have huge potential for development.