Stereospecific analyses of seed triacylglycerols from high-erucic acid brassicaceae: Detection of erucic acid at thesn-2 position inBrassica oleracea L. Genotypes |
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Authors: | David C Taylor Samuel L MacKenzie Alan R McCurdy Peter B E McVetty E Michael Giblin Eric W Pass Scot J Stone Rachael Scarth S Roger Rimmer Mark D Pickard |
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Affiliation: | (1) Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Place, S7N 0W9 Saskatoon, SK, Canada;(2) Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0 Saskatoon, Saskatchewan, Canada;(3) Department of Plant Science, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada;(4) Saskatchewan Wheat Pool, S7N 3R2 Saskatoon, Saskatchewan, Canada |
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Abstract: | Stereospecific analyses of triacylglycerols from selected high-erucic acid breeding lines or cultivars ofBrassica napus L. andB. oleracea L. have been performed. Initial lipase screening revealed that while allB. napus lines contained little or no erucic acid at thesn-2 position, several of theB. oleracea lines had significant proportions of erucic acid at this position. Detailed stereospecific analyses were performed on the
triacylglycerols from these lines by using a Grignard-based deacylation, conversion of thesn-1,sn-2 andsn-3 monoacylglycerols to their di-dinitrophenyl urethane (DNPU) derivatives, resolution of the di-DNPU-monoacylglycerols (MAGs)
by high-performance liquid chromatography on a chiral column, transmethylation of eachsn-di-DNPU MAG fraction and analysis of the resulting fatty acid methyl esters by gas chromatography. The findings unequivocally
demonstrate for the first time that, within the Brassicaceae, there existsB. oleracea germplasm containing seed oils with substantial erucic acid (30–35 mol%) at thesn-2 position. This has important implications for biotechnology and breeding efforts designed to increase the levels of erucic
acid in rapeseed beyond 66 mol% to supply strategic industrial feedstocks. In the first instance, the germplasm will be of
direct use in retrieving a gene encoding aBrassica lyso-phosphatidic acid acyltransferase with an affinity for erucoyl-CoA. In a breeding program, the germplasm offers promise for
the introduction of this trait intoB. napus by interspecific hybridization and embryo rescue. |
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Keywords: | Brassica napus Brassica oleracea erucic acid lyso-phosphatidic acid acyltransferase sn-2 position stereospecific analysis triacylglycerols |
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