垃圾渗滤液深度处理功能菌的筛选及应用

为了改善垃圾渗滤液生化处理系统尾水有机污染物的进一步降解效果,以垃圾渗滤液生化处理曝气池污泥为菌种分离源,用渗滤液生化尾水和琼脂调配培养基,采用生物强化技术驯化和筛选出3种功能菌,经16S rDNA鉴定为海杆菌属（Marinobacter）、不动杆菌属（Acinetobacter）和埃希式菌属（Escherichia）。将功能菌扩大培养,用物理循环吸附法投放于生物活性炭（BAC）反应器中。通过对照实验发现,自然挂膜的BAC仅对垃圾渗滤液生化尾水中分子量M为10-5 kDa的有机污染物具有较好的降解能力,而投加了功能菌的BAC对分子量M为100-30 kDa的有机污染物去除率为76.1%,对M〉100 kDa的有机污染物去除率为80.9%。投加功能菌的BAC可以提高垃圾渗滤液的生化处理效果。

Screening and Application of Functional Bacteria in the Advanced Treatment Process of Landfill Leachate

To improve organic pollutant degradability of landfill leachate,culture medium was prepared with landfill leachate and agar.And then three functional bacteria were cultured and screened from sludge in aeration pool of biochemical treatment system by bioaugmentation.Based on 16S rDNA identification,these bacteria were named Mari-nobacter（coded Y1）,Acinetobacter（coded Y2） and Escherichia（coded Y3）.Functional bacteria were scaling up,and then inoculated into biological-activated carbon（BAC） reactor by physical circle absorption method.With the control experiment,it was shown that the natural start-up BAC had significant degradation capacity to organic pollutants with molecular weight M of 10 ~ 5 kDa.And the inoculums＇ start-up BAC with functional bacteria was kept with was with removal rate of 76.1% for organic pollutants with molecular weight M of 100 ~ 30 kDa,and 80.9% for organic pollutants with molecular weight M exceeding 100 kDa.