Structural and functional changes of silk fibroin scaffold due to hydrolytic degradation |
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| Authors: | Mehdi Farokhi Fatemeh Mottaghitalab Jamshid Hadjati Ramin Omidvar Mohammad Majidi Amir Amanzadeh Mahmoud Azami Seyed Mohammad Tavangar Mohammad Ali Shokrgozar Jafar Ai |
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| Affiliation: | 1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran;3. Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran;4. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran |
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| Abstract: | In this study, hydrolytic degradation of silk fibroin (SF) in Phosphate buffer saline (PBS) after 12 weeks incubation was investigated. Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and X‐ray diffraction (XRD) patterns have confirmed the transition from crystalline β‐sheet to random coil in treated SF. A decrease in adhesion force and surface Young's modulus were observed using atomic force microscopy (AFM). Structural changes were further confirmed using scanning electron microscopy (SEM). Biocompatibility and alkaline phosphatase production of osteoblast cells were decreased significantly in treated SF scaffold. Moreover, a significant decrease in mRNA level of collagen type I and osteopontin compared with fresh SF scaffold was observed. Finally, structural and biological characteristics of SF scaffold could alter in PBS. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131, 39980. |
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| Keywords: | degradation biocompatibility properties characterization |
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