Quetol 651: Not just a low viscosity resin |
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Authors: | E Ann Ellis |
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Affiliation: | Consultant in Biological Electron Microscopy, Thomasville, Gerogia |
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Abstract: | Quetol 651, a low viscosity epoxy resin, is miscible with alcohols, acetone, and water. It is versatile and can be used as a single epoxide or mixed with other epoxides and anhydrides. The most important characteristic is that the addition of Quetol 651 to a formulation results in a lower viscosity embedding medium and allows for good detection of antigenic activity. Properly formulated and mixed resins containing Quetol 651 have excellent sectioning properties and good beam stability. The decrease in viscosity lends to lower specific gravity of the embedding medium and less interfering electron density between specimen elements resulting in better spatial resolution. New formulations and viscosity data are presented and compared to long used, embedding formulations and the extensive uses of Quetol 651 are reviewed. Microsc. Res. Tech. 79:50–57, 2016. © 2015 Wiley Periodicals, Inc. |
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Keywords: | low viscosity resin formulations postembedding immunocytochemistry |
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