Making the practically impossible “Merely difficult”—Cryogenic FIB lift‐out for “Damage free” soft matter imaging |
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| Authors: | Christopher DJ Parmenter Michael W Fay Cheryl Hartfield Hoda M Eltaher |
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| Affiliation: | 1. Nottingham Nanoscale and Microscale Research Centre, University of Nottingham, United Kingdom;2. Oxford Instruments Nanoanalysis, Concord, MA;3. Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), School of Pharmacy, University of Nottingham, United Kingdom |
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| Abstract: | The preparation of thinned lamellae from bulk samples for transmission electron microscopy (TEM) analysis has been possible in the focussed ion beam scanning electron microscope (FIB‐SEM) for over 20 years via the in situ lift‐out method. Lift‐out offers a fast and site specific preparation method for TEM analysis, typically in the field of materials science. More recently it has been applied to a low‐water content biological sample (Rubino 2012). This work presents the successful lift‐out of high‐water content lamellae, under cryogenic conditions (cryo‐FIB lift‐out) and using a nanomanipulator retaining its full range of motion, which are advances on the work previously done by Rubino (2012). Strategies are explored for maintaining cryogenic conditions, grid attachment using cryo‐condensation of water and protection of the lamella when transferring to the TEM. Microsc. Res. Tech. 79:298–303, 2016. © 2016 Wiley Periodicals, Inc. |
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| Keywords: | cryo‐FIB cryo‐lift‐out cryo‐TEM preparation soft‐matter frozen‐hydrated biological‐EM |
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