应用气相色谱-NPD测定烟叶中烟草特有亚硝胺

烟草特有亚硝胺(TSNA)是烟草中一类致癌性很强的化合物，对其进行准确的定量分析非常必要。笔者发展了已有的烟叶中烟草特有亚硝胺的萃取、纯化、浓缩和检测技术，开发出了一种应用气相色谱配备氮磷检测器分离并检测烟叶中NNN、NAT、NAB、NNK4种烟草特有亚硝胺的方法。主要过程包括烟样的超声波萃取，萃取液在硅藻土层析柱上层析，洗脱液的浓缩及应用气相色谱-NPD进行检测。该方法可以将极难分离的NAT-NAB分开，并具有较为简便、重复性好、可以同时处理多个样品的特点。

Determination of tobacco-specific nitrosamines in tobacco by GC-NPD

The tobacco-specific nitrosamines(TSNA)were highly carcnoigenic components in tobacco.How to determinate them precisely was very important.The method of extraction,purification,concentration and detection of tobacco-specific nitrosamines from tobacco was developed in this paper.NNN,NAT,NAB and NNK in flue-cured tobacco were separated and determinated by gas chromatography with a N-P detec-tor in this developed method.The procedure involved extraction of TSNA from tobacco by sonication,elu-tion of extract in a Kieselguhr extraction column ,concentration of elute and determination of TSNA by gas chromatography with a N-P detector.This method was shown to be simple in the determination of TSNA and shown good repeatability.NAT-NAB pair could be separated and was capable of performing many analyses per day on a variety of tobacco samples in this procedure.