暴露高活性晶面的TiO2纳米管的制备及生物活性

采用多次阳极氧化技术,在金属钛表面制备(001)晶面族择优生长的TiO_2纳米管阵列,研究电解液成分对锐钛矿TiO_2不同晶面相对比例的影响并考察其生物学活性。采用扫描电镜(SEM)、X射线衍射(XRD)等方法分析纳米管阵列的形貌特征和晶体结构,通过生物矿化CaP盐的沉积和蛋白吸附实验评价样品晶面对生物活性的影响。结果表明:通过改变电解液中H_2O的含量,能够便捷调控锐钛矿TiO_2纳米管中不同晶面的相对比例。当H_2O含量为2%(体积分数)时,制备得到的TiO_2纳米管阵列(004)晶面的织构系数T_c(004)可达4.76。而具有(001)优势晶面族的TiO_2纳米管,在类人体环境中能够为生物矿化和蛋白吸附提供更多的活性位点,加速羟基磷灰石的沉积并增加蛋白吸附量,表现出更优异的生物学活性。

Preparation and bioactivity of TiO_2 nanotubes exposed with highly active facets

TiO2 nanotubes arrays with dominant(001)facets were fabricated by multi-anodic oxidation technology.The influence factor of the electrolyte composition on the ratio of different facets for anatase TiO 2 and the bioactivity were investigated.Besides,the characterization of the surface morphologies and crystal structures were analyzed by scanning electron microscope(SEM)and X-ray diffraction(XRD),etc.Finally,the bioactivity was estimated by the CaP salt deposition vis biomin-eralization process and protein adsorption experiment.The results show that the relative proportions of different facets in the TiO2 nanotubes can be adjusted by changing the content of H2O in the electrolyte.The(004)facet texture coefficient of TiO2 nanotubes arrays prepared in 2%H2O(volume fraction)electrolyte reaches up to 4.76.The TiO2 nanotubes with dominant(001)facets can accelerate the deposition of hydroxyapatite and raise the amount of protein adsorption in the humanoid environment by providing more active sites for biomineralization and protein adsorption.The TiO2 nanotubes with a higher proportion of(001)facets have the more excellent biological activity.