Pathways for fatty acid elongation and desaturation in Neurospora crassa |
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Authors: | Thomas A. McKeon Marta Goodrich-Tanrikulu Jiann-Tsyh Lin Allan Stafford |
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Affiliation: | (1) Western Regional Research Center, USDA, ARS, 800 Buchanan St., 94710 Albany, CA |
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Abstract: | Neurospora crassa incorporated exogenous deuterated palmitate (16∶0) and 14C-labeled oleate (18∶1Δ9) into cell lipids. Of the exogenous 18∶1Δ9 incorporated, 59% was desaturated to 18∶2Δ9,12 and 18∶3Δ9,12,15. Of the exogenous 16∶0 incorporated, 20% was elongated to 18∶0, while 37% was elongated and desaturated into 18∶1Δ9, 18∶2Δ9,12, and 18∶3Δ9,12,15. The mass of unsaturated fatty acids in phospholipid and triacylglycerol is 12 times greater than the mass of 18∶0. Deuterium label incorporation in unsaturated fatty acids is only twofold greater than in 18∶0, indicating a sixfold preferential use of 16∶0 for saturated fatty acid synthesis. These results indicate that the release of 16∶0 from fatty acid synthase is a key control point that influences fatty acid composition in Neurospora. |
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