| 栀子黄A238nm/A440nm 值与西红花苷和栀子苷相关性研究 |
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| 引用本文： | 陈阳,张浩,蔡乐,李西兰,蒋李娟,许辉川,王文君.栀子黄A238nm/A440nm 值与西红花苷和栀子苷相关性研究[J].食品科学,2010,31(4):77-80. |
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| 作者姓名： | 陈阳 张浩 蔡乐 李西兰 蒋李娟 许辉川 王文君 |
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| 作者单位： | 1.遵义医学院珠海校区化学教研室 2.四川大学华西药学院生药学教研室 |
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| 基金项目： | 贵州省科学技术基金项目(黔科合J字[2008]2315号) |
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| 摘 要： | 目的：采用TLC、HPLC 和紫外分光光度法(UV)研究栀子黄OD 值(栀子黄中栀子苷的最大吸光度A238nm 与西红花苷的最大吸光度A440nm 的比值)与栀子苷含量的关系。方法：采用硅胶柱层析从栀子中分离西红花苷纯品，然后分别采用TLC、HPLC 和UV 来定性和定量分析栀子乙醇提取部位和经大孔吸附树脂精制的栀子黄，并计算波长238nm 和440nm 处的百分比吸光系数及OD 值。结果：经过对以上数据的分析，发现西红花苷本身在波长238nm处有明显紫外吸收，采用OD 值来控制栀子黄中的栀子苷将高估栀子苷含量。结论：栀子黄中栀子苷的控制不宜采用OD 值作为评价指标，而应采用具有分离功能的高效液相色谱法来作为质量控制的手段。
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| 关 键 词： | 栀子黄 西红花苷 栀子苷 UV TLC HPLC |
| 收稿时间： | 2009-04-16 |
Relationship between A_(238nm)/A_(440nm) Value of Gardenia Yellow and Contents of Crocin and Genipin |
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| CHEN Yang,ZHANG Hao,CAI Le,LI Xi-Lan,JIANG Li-Juan,XU Hui-Chuan,WANG Wen-Jun.Relationship between A_(238nm)/A_(440nm) Value of Gardenia Yellow and Contents of Crocin and Genipin[J].Food Science,2010,31(4):77-80. |
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| Authors: | CHEN Yang ZHANG Hao CAI Le LI Xi-Lan JIANG Li-Juan XU Hui-Chuan WANG Wen-Jun |
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| Affiliation: | 1. Department of Chemistry, Zunyi Medical College Zhuhai Campus, Zhuhai 519041, China；
2. Department of Pharmacognosy, West China School of Pharmacy, Sichuan University, Chengdu 610041, China |
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| Abstract: | TLC,HPLC and UV techniques were used to investigate the relationship between OD value (defined as the ratio of maximum absorbance value at 238 nm wavelength of genipin to maximum absorbance value at 410 nm wavelength of crocins) of gardenia yellow and genipin and crocins contents in it.Compounds with high purity were isolated from gardenia yellow by gel column chromatography and then subjected to qualitative and quantitative analysis by TLC,HPLC and UV.In addition,E1cm238,E1cm440 and OD values were calculat... |
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| Keywords: | gardenia yellow crocins genipin UV TLC HPLC 1 % 1 % |
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