The use of enzyme-linked immunosorbent assays (ELISA) for the determination of pollutants in environmental and industrial wastes. |
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Authors: | M Hirobe Y Goda Y Okayasu J Tomita H Takigami M Ike H Tanaka |
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Affiliation: | Japan EnviroChemicals, Ltd., 2-17-85 Juso-Honmachi Yodogawa-ku, Osaka 532-0024, Japan. hirobe_masato@jechem.co.jp |
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Abstract: | Twelve enzyme-linked immunosorbent assays (ELISA), for the determination of surfactants linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and alkylphenol ethoxylates (APE)], endocrine disruptors alkylphenol (AP), AP + APE, and bisphenol A (BPA)], estrogens 17beta-estradiol (E2), estrone (El), estrogen (ES: El + E2 + estriol (E3)), 1 7alfa-ethynylestradiol (EE2)], dioxins and polychlorinated biphenyls (PCBs), were validated on environmental water and industrial wastes. The lowest quantification limits of these ELISAs were 0.05 microg/L (BPA, E2, El, ES and EE2), 2 microg/L (AE), 3 microg/L (dioxins and PCBs), 5 microg/L (AP, AP + APE) and 20 microg/L (LAS and APE). To apply these ELISAs to environmental or industrial waste samples, simple and appropriate pre-treatment methods were also developed for each ELISA. With optimized pre-treatments, the values of ELISAs were well co-related, in all cases, to those of instrumental analytical methods such as liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-resolution gas chromatography mass spectrometry (HR-GC-MS), etc. |
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