| 藠头中抗菌活性成分的抑癌作用及机理研究 |
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| 引用本文： | 孙运军,柏建山,陈宇,孟松,谢伟岸,丁学知,莫湘涛,夏立秋.藠头中抗菌活性成分的抑癌作用及机理研究[J].食品科学,2004,25(11):295-299. |
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| 作者姓名： | 孙运军 柏建山 陈宇 孟松 谢伟岸 丁学知 莫湘涛 夏立秋 |
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| 作者单位： | 湖南师范大学生命科学学院,湖南,长沙,410081 |
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| 基金项目： | 湖南省重点攻关项目(03SSY3107) |
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| 摘 要： | 从藠头(Allium chinense G.Don)中提取的活性物质在不同温度处理下具有良好的热稳定性。利用其活性物质处理肝癌(HepG-2)细胞和海拉(Hela)细胞,发现该物质对两种癌细胞生长都有抑制作用,且呈浓度依赖性。采用流式细胞仪分析发现,该物质可改变两种癌细胞的细胞周期,但不诱导细胞凋亡。通过体外对木瓜蛋白酶和蛋白酶K活力的测定,该物质对木瓜蛋白酶活力有较大的影响,对蛋白酶K活力影响不明显,这可能与木瓜蛋白酶的活性中心含巯基有关。分析认为藠头活性成分的抑癌作用可能是通过影响癌细胞内象木瓜蛋白酶等一些活性中心含巯基的酶的活力来实现的。
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| 关 键 词： | 藠头 抗菌活性成分 抑癌作用 细胞周期 木瓜蛋白酶 |
| 文章编号： | 1002-6630(2004)11-0295-05 |
| 修稿时间： | 2004年7月16日 |
Anticancer Effects and Possible Mechanism of Antibacterial Components from Allium chinense |
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| SUN Yun-jun,BAI Jian-shan,CHEN Yu,MENG Song,XIE Wei-an,DING Xue-zhi,MO Xiang-tao,XIA Li-qiu.Anticancer Effects and Possible Mechanism of Antibacterial Components from Allium chinense[J].Food Science,2004,25(11):295-299. |
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| Authors: | SUN Yun-jun BAI Jian-shan CHEN Yu MENG Song XIE Wei-an DING Xue-zhi MO Xiang-tao XIA Li-qiu |
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| Affiliation: | SUN Yun-jun,BAI Jian-shan,CHEN Yu,MENG Song,XIE Wei-an,DING Xue-zhi,MO Xiang-tao,XIA Li-qiu* |
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| Abstract: | This study showed that the antibacterial components from Allium chinense kept stable under different temperature.After treated by the components, the proliferation of HepG-2 and Hela cells were inhibited and their cell cycle distribution werechanged. Analysis revealed that the components didn't induce apoptosis. The components had more inhibitive effect on the enzymeactivity of papain than on proteinase K in vitro. The possible anticancer mechanism of the components was that the activity of somemercapto enzymes such as papain in cancer cells were inhibited by the components. |
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| Keywords: | Allium chinense antibacterial components anticancer cell cycle distribution papain |
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