| 凝胶萃取浓缩蛋白质及过程优化 |
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| 引用本文： | 王世昌 解利昕. 凝胶萃取浓缩蛋白质及过程优化[J]. 化工学报, 1990, 41(1): 74-80 |
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| 作者姓名： | 王世昌 解利昕 |
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| 作者单位： | 天津大学化学工程研究所,天津大学化学工程研究所,天津大学化学工程研究所,天津大学化学工程研究所 天津,天津,现在国家海洋局天津海水淡化与综合利用研究所工作,天津,天津 |
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| 摘 要： | 研究了淀粉—聚丙烯酰胺接枝共聚物等三种凝胶吸水溶胀和在某一酸度条件下收缩释水的过程.利用凝胶这一酸敏效应可以构成萃取循环.以浓缩溶液中的蛋白质或其他大分子物质.文中还讨论了溶液中离子种类、溶质浓度和凝胶粒度等对其溶胀、收缩性能的影响.在对实验数据进行回归分析的基础上,建立了按复合形法进行计算的优化方法,得出实际分离过程的最佳溶胀—收缩范围.
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| 关 键 词： | 凝胶 萃取 蛋白 浓缩 |
Gel-Extraction for Protein Concentration and Process Optimization |
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| Wang Shichang,Xie Lixin,Jin Lei and Yu Guocong. Gel-Extraction for Protein Concentration and Process Optimization[J]. Journal of Chemical Industry and Engineering(China), 1990, 41(1): 74-80 |
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| Authors: | Wang Shichang Xie Lixin Jin Lei Yu Guocong |
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| Affiliation: | Wang Shichang,Xie Lixin,Jin Lei and Yu Guocong(K. T. Yu)(Chemical Engineering Research Center,Tianjin University,Tianjin) |
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| Abstract: | The polymer gels of H-SPAM, PAA-Na and S-PAA-Na can swell in water and collapse suddenly at a given pH, with the water released. By using them as extractants, a new process for protein concentration has been developed. The properties of acid-sensitive gel, the influences of ions, solute concentration and gel size on the performance of gel-extraction were studied experi-mentally.Optimization of the acid-sensitive gel-extraction process with complex method has been developed on the basis of regression oi experimental data. |
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