固相萃取净化-离子色谱法测定水产品中的6种阴离子含量

目的 测定水产品中6种无机阴离子含量.方法 采用超声提取、固相萃取柱净化的方法对试样进行前处理,高容量阴离子交换色谱柱分离,离子色谱抑制型电导检测器检测.结果 F-、NO2-在0.1～10.0 mg/L,C1-、NO3-、pO3-4、SO24在1.0～50.0 mg/L范围内线性良好,回收率在89.8％～103.0％之间,相对标准偏差在3.5％ ～5.7％之间,检出限分别为F-:0.6 mg/kg; CI-:1.0 mg/kg; NO/-:2.0 mg/kg;NO3-:1.6 mg/kg; PO3-4:4.0 mg/kg;SO2-4:1.4 mg/kg.结论 本方法准确、灵敏、操作简便,一次进样15 min内完全分离,适用于水产品中6种无机阴离子含量的检测.

Determination of six anions in aquatic products by SPE-Ion chromatography

Objective To develop a method for simultaneous determination of six anions in aquatic products by ion chromatography. Methods Food samples were pre-treated by ultrasonic extraction, high speed centrifugation and solid phase extract clean up, and the resulting supernatants were injected into a high-capacity anion exchange column for separation and detected by suppressed conductivity. Results The linearity of F^- and NO2^- were satisfying in the range of 0.1~10.0 mg/L. The linearity of Cl^-, NO^3-, PO3^-4, and SO2^-4 were good in the range of 1.0~50.0 mg/L. The recovery of the method was 89.8~103.0% and relative standard deviation (RSD) was 3.5~5.7%. The detection limits were F^-: 0.6 mg/kg; Cl^-: 1.0 mg/kg; NO2^-: 2.0 mg/kg; NO3^-: 1.6 mg/kg; PO3^-4: 4.0 mg/kg; SO2^-4: 1.4 mg/kg. Conclusion The method could simultaneously and completely separate all anions in 15 minutes with good accuracy, sensitivity, simplicity and could be applied for inspection of F^-, Cl^-, NO2^-, NO3^-, PO3^-4 and SO2^-4 in aquatic products.