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Biochemical properties of skin collagens isolated from black carp (Mylopharyngodon piceus) |
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| Authors: | Yuanjun Jia Haibo Wang Haiyin Wang Yunyan Li Min Wang Jian Zhou |
| Affiliation: | 2. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China 1. Department of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan, Hubei, China 3. Tongji Medical Collage, Huzhong University of Science and Technology, Wuhan, Hubei, China |
| Abstract: | Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted with the yield of 15.5 and 26.5% on the basis of dry weight from the skin of black carp (Mylopharyngodon piceus). ASC and PSC were similar in amino compositions and subunit compositions, but they were slightly different in the secondary structure. Both of ASC and PSC, either rehydrated in distilled water or in acetic acid, had a lower denaturation temperature than pig skin collagen (PPSC), but their denaturation temperature were higher than that of collagens from other freshwater fish. Intrinsic viscosity of different collagens decreased in the order of PPSC>PSC>ASC. Both of ASC and PSC showed only partial degradation in collagenase solution, but they revealed more sensitive to collagenase compared with PPSC. Black carp skin collagens did not induce a significant cytotoxic effect according to the results of in vitro cytotoxicity test. |
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