| Let-7a在UVB照射的HaCaT细胞中的表达 |
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| 引用本文： | 郭玲,何英杰,郑莉,吕超,丁振华. Let-7a在UVB照射的HaCaT细胞中的表达[J]. 辐射研究与辐射工艺学报, 2009, 27(6): 360-364 |
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| 作者姓名： | 郭玲 何英杰 郑莉 吕超 丁振华 |
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| 作者单位： | 南方医科大学公共卫生与热带医学学院放射医学系,广州,510515 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 研究let-7a在UVB照射后的人角质细胞HaCaT中的表达。HaCaT细胞受UVB照射后继续培养2h、4h、8h、12h、24h，用real-timeqPCR检验let-7a的表达水平。利用在线数据库PicTar等预测靶基因并用Gostat软件对靶基因进行功能分类。结果发现let-7a在照射后12h出现表达水平的高峰（大于7倍），而在其它时间点没有改变。在对其靶基因预测后用Gostat进行功能分类后发现，casp3、bc1212、map3kl和cdk5等基因同时也是UVB诱导的细胞周期调节的靶基因。这些结果提示let-7a可能在UVB致损伤的机制中起关键作用。
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| 关 键 词： | Let-7a HaCaT 紫外线B |
The expression level of let-7a in UVB irradiated HaCaT cells |
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| GUO Ling,HE Yingjie,ZHENG Li,L Chao,DING Zhenhua. The expression level of let-7a in UVB irradiated HaCaT cells[J]. Journal of Radiation Research and Radiation Processing, 2009, 27(6): 360-364 |
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| Authors: | GUO Ling HE Yingjie ZHENG Li L Chao DING Zhenhua |
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| Affiliation: | GUO Ling HE Yingjie ZHENG Li Lü Chao DING Zhenhua (Department of Radiation Medicine,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | In order to study the expression level of let-7a in HaCaT exposed to UVB real-time qPCR was used to detect the expression level of let-7a at 2 h, 4 h, 8 h, 12 h and 24 h after UVB irradiation. Online database PicTar can be used to predict the targets of let-7a and performed function categories with softerware Gostat. The results show that the expression level of let-7a at 12h is higher than that at other time points with more than 7 times. The target prediction and Gostat analysis indicate that casp3、bcl2l2、map3k1 and cdk5 may be UVB-induced target genes of cell cycle regulation. These results suggest that let-7a may play a key role in the mechanism of UVB-induced injury. |
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| Keywords: | Let-7a HaCaT Let-7a HaCaT Ultraviolet B |
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