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miR-373-3p Regulates Invasion and Migration Abilities of Trophoblast Cells via Targeted CD44 and Radixin
Authors:Hyun-Jung Lee  Seung Mook Lim  Hee Yeon Jang  Young Ran Kim  Joon-Seok Hong  Gi Jin Kim
Affiliation:1.Department of Biomedical Science, CHA University, Seongnam 13488, Korea; (H.-J.L.); (S.M.L.); (H.Y.J.);2.CHA Bundang Medical Center, Department of Obstetrics and Gynecology, Seongnam 13496, Korea;3.Department of Obstetrics and Gynecology, Daerim St. Mary’s Hospital, Seoul 07442, Korea;4.Research Institute of Placental Science, CHA University, Seongnam 13488, Korea
Abstract:Preterm labor (PTL) is one of the obstetric complications, and is known to be associated with abnormal maternal inflammatory response and intrauterine inflammation and/or infection. However, the expression of specific miRNAs associated with PTL is not clear. In this study, we performed combination analysis of miRNA array and gene array, and then selected one miRNA (miR-373-3p) and its putative target genes (CD44 and RDX) that exhibited large expression differences in term and PTL placentas with or without inflammation. Using qRT-PCR and luciferase assays, we confirmed that miR-373-3p directly targeted CD44 and RDX. Overexpression of miR-373-3p reduced the migration and invasion of trophoblast cells, while inhibition of miR-373-3p restored the migration and invasion abilities of trophoblast cells. Finally, we validated the expression of miR-373-3p and its target genes in clinical patients’ blood. miR-373-3p was increased in PTL patients’ blood, and was the most expressed in PTL patients’ blood with inflammation. In addition, by targeting the miR-373-3p, CD44 and RDX was decreased in PTL patients’ blood, and their expression were the lowest in PTL patients’ blood with inflammation. Taken together, these findings suggest that miR-373-3p and its target genes can be potential biomarkers for diagnosis of PTL.
Keywords:preterm labor   miR-373-3p   CD44   radixin   invasion   trophoblast cells
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