在搅拌培养和静态培养中造血细胞亚群组成的分析

目的　分析转瓶和方瓶培养过程中造血细胞亚群组成的变化。方法　观察培养过程中脐血单个核细胞总细胞扩增倍数 ,CD34+ 细胞、CD33+ 细胞、CFU GM、CD4 1+ 细胞、CFU Mk和BFU E占总细胞的比例及其随培养时间的变化。结果　 14d的培养中 ,无论是用转瓶和方瓶 ,总细胞不断扩增 ,CD34+ 细胞含量在第 7天、CFU GM含量在第 10天、BFU E含量在第 5天达到最高 ,而后出现分化 ,转瓶和方瓶培养的CD34+ 细胞和CFU GM和BFU E含量差异无显著意义 ;CFU Mk则不同 ,其含量在转瓶培养第 7天时达到最高 ,而在方瓶第 10天达到最高 ,在转瓶中CFU Mk的含量一直低于方瓶培养 ;两种培养过程中CD33+ 细胞比例均在 5 0 %～ 70 %之间 ,而且差异无显著意义。转瓶培养中CD4 1+ 细胞含量到后期出现下降 ,而方瓶中则一直增加。结论　与静态培养环境相比 ,搅拌环境对干 祖细胞分化速度、粒 巨噬系祖细胞和红系祖细胞的分化没有明显影响 ,但不利于巨核系祖细胞的扩增 ,促进了其分化

Subgroups of Hematopoietic Cells Cultured in T-flask and in Spinner Flask

Objective To analyze the change of subgroups of hematopoietic cells during culture in T-flask and in spinner flask.Methods Observe the expansion fold of total cells, the proportions of CD34~+, CD33~+, CFU-GM, CD41~+ cells, CFU-Mk and BFU-E in the total cells and the change of the proportion as time goes on. Results During the 14-day culture, both in spinner flask and in T-flask, the expansion folds of total cells increased continuously. The contents of CD34~+ cells, CFU-GM and BFU-E reached peak values at the 7~(th), the 10~(th) and the 5~(th) day after culture respectively. However, the contents of the cells in spinner flask and in T-flask showed no significant difference. CFU-Mk cell content reached a peak value at the 7~(th) day after culture in spinner flask and at the 10~(th) day in T-flask, and its content in spinner flask was significantly lower than that in T-flask all the time.Both the proportions of CD33~+ cells in spinner flask and in T-flask were 50%-70% and showed no significant difference.The CD41~+ cell content decreased at the late stage of culture in spinner flask but increased all the time in T-flask.Conclusion Compared with that in T-flask, the culture in spinner flask showed no significant influence on the differentiation of stem cell/progenitor cell, granulocyte/macrophage progeniotors and erythrocyte progenitors. However, it decreased the expansion and accelerated the differentiation of metgakarocyte progenitors.